Summary
Type: SPE
Application #: 305610
Catalog#: 730005 - SPE Cartridges, Chromabond, C18, 6ml, PP, 1000mg, 30/pk
Detection:
Conditions: Column conditioning: 1 column volume methanol – water – formic acid (90:9:1, v/v/v)
(Do not let run the column dry.)
Sample aspiration: 0.8 mL of pretreated sample
Elution: 9 mL methanol – water – formic acid (90:9:1, v/v/v)
Evaporation: to dryness (40 °C)
Reconstitution: 1 mL methanol – acetonitrile – water – formic acid (15:5:75.25:4.75, v/v/v/v); filtration with 0.2 µm filter before injection into UHPLC
Authors: Dias, A.L.S. et al.
Source: J. Chromatogr. B 907 (2012) 108–116